T4 GP32 Protein

T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.

Product Source

E. coli cells expressing T4 gp32 gene.

Product Includes

• T4 GP32 protein
• 10X GP32 reaction buffer

10X GP 32 reaction buffer composition

200 mM Tris-acetate

1M Potassium acetate

100 mM Magnesium acetate

10 mM DTT

pH 7.8 @ 25ºC

Storage Temperature: -20ºC

Heat Inactivation: 65°C for 20 min

Quality Control assays

T4 GP32 protein is free from detectable nuclease contamination.