FOCUS™ Protein Solubilization Buffer is a dry, urea-based and ready-to-use buffer for protein solubilization. It is a proprietary modification of urea based chaotropic extraction buffers. Normal urea based buffers solubilize proteins effectively however can modify the native charge of the proteins, due to carbamylation, a process that modifies amino groups, inducing changes in the isoelectric point of proteins leading to artifactual 2D results.
FPS Buffer is a DRY, pre-mixed formulation of urea, thiourea, CHAPS and non detergent sulfobetaine (ND SB 201) for maximum solubilizing strength. The dry format allows researcher’s to freshly rehydrate as much or as little as is required and therefore prevent urea induced carbamylation.
