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BCA Protein Assay (60 Citations)

Catalog Numbers: 786-570 Bicinchoninic Acid (BCA) Protein Assay 500 Assays/ 2500 Micro-assays 786-571 Bicinchoninic Acid (BCA) Protein Assay 1,000 Assays/ 5,000 Micro-assays 786-890 Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard 500 Assays/ 2500 Micro-assays 786-891 Bicinchoninic Acid (BCA) Protein Assay with Non Animal Protein Standard 1,000 Assays/ 5,000 Micro-assays 786-844 BCA Solution (BCA Reagent A) 250ml 786-845 BCA Solution (BCA Reagent A) 500ml 786-846 BCA Solution (BCA Reagent A) 1L 786-847 BCA Solution (BCA Reagent A) 1gal 786-848 Copper Solution (BCA Reagent B) 25ml BCA Protein Assay 94
BCA Protein Assay
BCA Protein Assay

The BCA Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid (BCA) chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562 nm that is linear for increasing protein concentrations between the range of 0.02-2mg/ml. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations. The Bicinchoninic Acid (BCA) Protein Assay is suitable for quantifying protein solutions in 1ml assays or in micro-wells.

Features

  • Sensitive colorimetric assay
  • Linear range of 20-2,000µg/ml
  • Compatible with wide range of ionic and non-ionic detergents
  • 1ml cuvette or micro well compatible

Applications

  • For the estimation of protein concentration
The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.
 
For dilute protein samples (0.5-20µg/ml), we recommend the Micro Bicinchoninic Acid (BCA) Protein Assay. 
 
For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.
Protocol
786-570
786-571
786-890
786-891
786-844
786-845
786-846
786-847
786-848
Material Safety Data Sheet
786-570
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-571
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-890
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-891
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-844
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-845
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-846
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-847
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-848
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
A Non-Animal Alternative to BSA Protein Standards
Bioassays Handbook
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Protein Assay Handbook & Selection Guide  An introduction to protein assays.
Certificate Of Analysis
786-571
192312
192905B
786-844
210806

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